|
|
|
| Registros recuperados: 18 | |
|
|
| Urashima, Tadasu; Nakamura, Tadashi; Ikeda, Akiko; Asakuma, Sadaki; Arai, Ikichi; Saito, Tadao; Oftedal, Olav T.; 浦島, 匡; 中村, 正. |
| Carbohydrates were extracted from a sample of milk from a mink, Mustela vison (Family Mustelidae). Free neutral and acidic oligosaccharides were isolated from the carbohydrate fraction and their chemical structures were compared with those of white-nosed coati (Nasua narica, Procyonidae) and harbour seal (Phoca vitulina, Phocidae) that we had studied previously. The ratio of free lactose to milk oligosaccharides was similar to that in milk of the white-nosed coati; in both species, this ratio was much lower than that in the milk of most eutherians. The neutral oligosaccharides of mink milk had alpha(1-3)-linked Gal or alpha(1-2)-linked Fuc residues at their non-reducing ends, as in the neutral oligosaccharides of white-nosed coati milk. Some of the neutral... |
|
Palavras-chave: Mink; Mustela vison; Mustelidae; Milk oligosaccharides; Neutral oligosaccharides; Sialyl oligosaccharides. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/729 |
| |
|
|
| Mori, Fumiki; Nakamura, Tadashi; Nakamura Osawa, Aya; Arai, Ikichi; Urashima, Tadasu; 森, 史樹; 中村, 正; 男澤－中村, 綾; 荒井, 威吉; 浦島, 匡. |
| Cell wall polysaccharides were separated from Leuconostoc mesenteroides subsp. cremoris isolated from “Filmjolk"，a fermented milk made commercially in Sweden. Two Components(CNPI and CNP2) were further purified by gelfiltration on HPLC and the molecu1ar weights were determined to be 850，000 and 81，000. CNP-2 was shown to contain two polysaccharides in major and minor by an lH-NMR. The major polysaccharide was shown to have a heptasaccharide repeating unit which contained five α and two β anomers，and also contained non reducing galactopyranose，O-2，3 disubstituted glucopyranose，O-3 substituted galactopyranose and O-3，6 disubstituted galactopyranose as units in a molar ratio of 3:1:1:2. On the other hand，the minor polysaccharide was shown to have a... |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2921 |
| |
|
|
| 荒井, 威吉; 仙田, 晶嗣; 山上, 貴礼; 無量谷, 亜希; 中村, 正; 浦島, 匡; ARAI, lkichi; SENDA, Akitsugu; YAMAGAMI, Kihiro; MURYOYA, Aki; NAKAMURA, Tadashi; URASHIMA, Tadasu. |
| The efficacy of the use of acidic electrolyzed water (AEW) for premilking teat cleaning and disinfection (TCD) was evaluated using a split-udder experimental design. The efficacy of AEW for TCD was compared with that of NaClO solution contained 200 ppm available chlorine (HClO). The viable cell counts levels of SPC，lactic acid bacteria，thermotrophilic bacteria and anaerobe (group A) which contaminated on teat skin were over 10³ cfu/cm²，whereas psychrotrophilic bacteria and coliforms (group B) were less 10² cfu/cm². After TCD with AEW and HClO，viable cell counts levels of the microorganisms of group A and group B were decreased to 2.8-7.6% and 10.0-14.5% respectively. On the other hand，the efficacy of AEW or HClO for TCD was almost constant throughout one... |
|
Palavras-chave: 強酸性電解水; 乳頭清拭; Acidic electrolyzed water; Premilking teat cleaning and disinfection. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2993 |
| |
|
|
| Ozawa, Takahiro; Acosta, Tomas J.; Nakamura, Tadashi; Miyamoto, Akio; 中村, 正; 宮本, 明夫. |
| The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate... |
|
Palavras-chave: GnRH; Immunoreactivity; Microdialysis; Follicle; Cow. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2970 |
| |
|
|
| NOZAKI, Hirofumi; WIJAYAGUNAWARDANE, Missaka P.B.; KODITUWAKKU, Suranga P.; YOSHIDA, Takashi; NAKAMURA, Tadashi; ARAI, Ikichi; URASHIMA, Tadasu; MIYAMOTO, Akio; 中村, 正; 浦島, 匡; 宮本, 明夫. |
| N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17β-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) α, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the... |
|
Palavras-chave: Bovine; Luteinizing hormone (LH); N-acetylglucosaminyltransferase I; Oviduct; Tumor necrosis factor (TNF) α; Vascular endothelial growth factor (VEGF). |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2978 |
| |
|
|
| 仙田, 晶嗣; 中村, 正; 浦島, 匡; 荒井, 威吉. |
| 生乳中の酵母の動態を明らかにすることを目的として，帯広畜産大学と近郊の酪農家3戸で飼養している健康なホルスタイン種乳牛（体細胞数1×105cfu/ml以下）の乳頭表皮，分房乳，バルク乳およびローリー乳中の酵母数の月別変動について調べた．各試料は1999年7月から2000年6月までの1年間，それぞれ毎月1回ずつ採取した．生乳中の酵母の検出率と平均酵母数はそれぞれ順に，乳頭表皮では70.1％，52cfu/cm2，分房乳は47.9％，1.8cfu/ml，バルク乳は100％，28cfu/mlおよびローリー乳では100％，66cfu/mlであった．乳頭表皮および各種類の生乳中において90％以上を占める酵母数の水準は，乳頭表皮が150cfu/cm2以下（90.8％），分房乳が10cfu/ml以下（94.4％），バルク乳が100cfu/ml以下（97.8％）およびローリー乳が150cfu/ml以下（91.7％）であると考えられた．生乳中の酵母数は夏から秋期に高く，冬から春期に低くなる季節的な変動傾向が認められた．健康な乳牛の分房乳中では，主な乳房炎起因菌（C. parapsilosis, C. lambica, C. catenulata, C. rugosa）は夏期に検出されたが，冬から春期には検出されなかった． The objective of this study was to investigate the seasonal incidences of yeast and the seasonal changes of yeast counts (cfu/ml) in a high quality raw milk produced by healthy Holstein cows (≤1×105 somatic... |
|
Palavras-chave: Yeast in milk; The incidence of yeast; The seasonal changes of yeast counts. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2640 |
| |
|
|
| Sumiyoshi, Wataru; Urashima, Tadasu; Nakamura, Tadashi; Arai, Ikichi; Nagasawa, Takashi; Saito, Tadao; Tsumura, Norihiko; Wang, Bing; Brand-Miller, Janette; Watanabe, Yoko; Kimura, Kazumasa; 住吉, 渉; 浦島, 匡; 中村, 正; 荒井, 威吉; 長澤, 孝志; 齋藤, 忠夫; 津村, 宣彦; 渡邉, 陽子; 木村, 一雅. |
| The concentrations of the six major sialyl oligosaccharides found in the milk of 24 Japanese women were determined using reverse phase high performance liquid chromatography of the 3-methyl-1-phenyl-5- pyrazolone derivatives of the oligosaccharides. The milk was collected at 4, 10, 30 and 100 days postpartum. The concentrations of all six oligosaccharides decreased during the course of lactation. The concentrations of 6'-sialyllactose, sialyllacto-N-tetraose b and disialyllacto-N-tetraose decreased only after 10 days postpartum, whereas those of sialyllacto-N-tetraose a and sialyllacto-N-neotetraose c as well as 3'-sialyllactose decreased from 4 days postpartum. Large differences were observed between present data and those previously reported for Italian... |
|
Palavras-chave: Human milk; Sialyl oligosaccharide; Lactation period; PMP; Reverse phase HPLC. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3287 |
| |
|
|
| 浦島, 匡; 上村, 祐介; 中村, 正; 佐々木, 基樹; Urashima, Tadasu; Uemura, Yuhsuke; Nakamura, Tadashi; Sasaki, Motoki. |
| ペンシルバニア紀(>3億1000万年前)に，有羊膜類であるSynapsidは他の系統から分化し，腺を発達させた。乳腺は毛包と会合した祖先型アポクリン腺から発達した。乳腺と毛の関係は単孔類の乳腺に保持されている。また，有袋類における乳腺毛の初期個体発生時の存在とその後の退化に，その証拠が残されている。単孔類の乳頭のない乳腺区分にあるmammo-ピロ-脂腺単位の密集した集団は，透水性を示す卵への水分や他の成分の補給のための構造的進化を反映しているかもしれない。ラクトースなどの成分は，初期Synapsidの祖先アポクリン腺から分泌されていたかもしれない。三畳紀のTherapsidは，複雑な栄養成分を含む乳を分泌し，卵サイズを小さくし，孵化時の新生子の未熟化を引き起こした。乳腺区分に生じた乳頭は，乳腺の毛を退化させた。 乳腺分泌は，羊皮状の卵殻をもつ卵を産むSynapsidで開始されたと考えられる。羊皮状の卵殻をもつ卵は，低湿度の空気にふれた際，急速に水分損失を被る。Synapsidは抱卵嚢の中で抱卵することで卵の乾燥を防いだが，これは母親の行動を制限した。羊皮状の卵殻をもつ卵は，殻を通して液体水分を補給することができる。乳腺分泌は，本来卵に水分を供給する手段として開始され，Cynodont (キノドン類)において内温性獲得の要因となった。Synapsidの卵は，ナトリウムやカルシウムイオンなどの栄養素を摂取した可能性がある。孔のある卵殻と2層性の卵黄膜は，子宮内での分泌物と抱卵時での乳腺分泌物の摂取を促進したと考えられる。 |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2924 |
| |
|
| |
|
|
| 荒井, 威吉; 中島, 景典; 丸山, 千弘玲; 中村, 正; 戸羽, 隆宏; 浦島, 匡; Arai, Ikichi; Nakajima, Keisuke; Maruyama, Chigure; Nakamura, Tadashi; Toba, Takahiro; Urashima, Tadasu. |
| Microflora of lactic acid bacteria and yeasts of Laban which had been made with bovine or ovine milk by traditional natural fermentation in Yemen，were studied. The mean of viable cell counts of microorganisms in Laban was 6.4 x10⁸ cfu/ml. The 60 strains of lactic acid bacteria and 24 strains of yeasts were isolated and identified. The percentages of strains on lactic acid bacteria flora were as follows: Lactococcus lactis subsp.lactis① was dominant in 88%. Lc. lactis subsp. lactis②，Leuconostoc mesenteroides subsp. mesenteroides，and Leuc. lactis were in 5，5，and 2%，respectively. It was recognized that the some characteristics of Lc. lactis subsp. lactis① of the dominant strain were different from those of Lc. lactis subsp.lactis②. The percentages of strains... |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2923 |
| |
|
|
| Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡. |
| The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose> α1-3α1-6 mannotriose>α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large... |
|
Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185 |
| |
|
| |
|
|
| 中村, 正; 菅井, 理子; 男澤, 綾; 有賀, 秀子; 小疇, 浩; キュラ, キーユキア; 荒井, 威吉; 浦島, 匡; Nakamura, Tadashi; Sugai, Michiko; Nakamura, Aya; Ariga, Hideko; Koaze, Hiroshi; Kiiyukia, Cura; Arai, Ikichi; Urashima, Tadasu. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1877 |
| |
| Registros recuperados: 18 | |
|
|
|
